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Can someone with more biological background correct me, but I once read that if a species drops below ~250 in population, that it is basically already eradicated. The remaining genpool is not diverse enough to develop further and stagnates on inbreeding when it dips below a certain threshold.
That rings a bell, but I thought there were some ways around it with highly selective breeding programs, and/or mixing in the genes of a closely related species (if one exists & is interbreedable)